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BMS204/3, Affymetrix, Human IL-8 Platinum ELISA 96 tests

The human IL-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-8. The human IL-8 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8.
Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes.

The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Reactivity Human
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, heparin)
Sensitivity 2.0 pg/mL
Standard Curve Range 15.6 – 1,000 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-8/NAP-1
Biotin-Conjugate anti-human IL-8/NAP-1 polyclonal antibody
Streptavidin-HRP
Human IL-8/NAP-1 Standard, 100 ng/mL
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Reported Applications ELISA

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