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Geneaid, Genomic DNA Mini Kit (Blood/Cultured Cell)

RM 746.50RM 2,042.80

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Description

The Genomic DNA Mini Kit (Blood/Cultured Cell)is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.

Advantages (Cat. # GB100, GB300)
  • Purify genomic DNA within 25 minutes!
  • High Yield, High Quality Genomic DNA: 4-10 μg of pure genomic DNA (A260/A280 = 1.8-2.0) from 300 µl of whole blood
  • Variety of Samples: up to 300 µl of whole blood, up to 200 µl of buffy coat, up to 1 x 107 cultured cells, up to 2 x 108 yeast and other fungus species
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C) for up to 1 year without showing any reduction in performance
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
  • RBC Lysis Buffer
  • GT Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • GD Columns
  • 2 ml Collection Tubes
Quality Control
The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA from 300 μl of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (4-10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Mini Kit (Blood/Cultured Cell) Functional Test Data

Figure 1. Genomic DNA (20-30 kb) was extracted from 300 µl of whole blood using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Sample Yield 260/280
1. 300 µl 8.07 µg 1.87
2. 300 µl 8.18 µg 1.87
3. 300 µl 8.19 µg 1.87

Additional information

No. of Reactions

100 rxns, 300 rxns

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