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The Genomic DNA Mini Kit (Tissue) is a genomicDNA Extraction Kit optimized for genomic, mitochondrial and virus DNA purification from a variety of animal tissue and insects. This genomic DNA extraction kit includes Micropestles to ensure efficient homogenization of tissue samples and to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the genomic DNA spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer, TE or water and is ready for use in a variety of downstream applications such as PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR. For RNA extraction from tissue samples, theCheap Cytotec Philippines is also available.

Advantages (Cat. # GT050, GT100, GT300)
  • Purify genomic DNA from tissue samples in 30 minutes!
    High Yield: 10-20 µg (0.5 cm mouse tail), 20-50 µg (20 mg mouse kidney)
  • Sample Size: up to 30 mg of tissue (tailsnips, liver, kidney, brain, earpunches, insects etc.)
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Provided Micropestles are certified DNase and RNase-free
  • Storage: dry at room temperature (15-25°C) for up to 1 year without showing any reduction in performance
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • Micropestle
  • GT Buffer
  • GBT Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Genomic DNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA from a 20 mg mouse liver sample is extracted using the Genomic DNA Mini Kit (Tissue). The purified DNA (10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Mini Kit (Tissue) Functional Test Data

Figure 1. Genomic DNA from a variety of tissue samples was extracted using the Genomic DNA Mini Kit (Tissue). The purified genomic DNA (20-30 kb) was EcoRI digested and analyzed by electrophoresis on a 1% agarose gel.
M =  Geneaid 1 Kb DNA Ladder
Mouse Tissue Tissue Size Total Yield
Tail 0.5 cm 10-20 µg
Liver 20 mg 10-20 µg
Brain 20 mg 10-20 µg
Lung 20 mg 5-10 µg
Muscle 20 mg 5-10 µg
Kidney 20 mg 20-50 µg

Additional information

No. of Reactions

100 rxns, 300 rxns, 50 rxns

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