Cusabio, Human Corticosterone,CORT ELISA Kit

RM 3,085.90

SKU: CSB-E07968h Categories: , ,

Description

CSB-E07968h, Human Corticosterone,CORT ELISA Kit, 96T,5×96T,10×96T

Product Name:

Human Corticosterone,CORT ELISA Kit

Product Type : ELISA Kit
Code : CSB-E07968h
Size : 96T,5×96T,10×96T
Abbreviation : CORT
Alias : N/A
Species : Human
Sample Types : serum, plasma.
Detect Range : 0.5 ng/ml -20 ng/ml
Sensitivity : 0.5 ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Research Area : Signal Transduction
Protocol :

 Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 103
Range % 99-108
1:2 Average % 92
Range % 88-96
1:4 Average % 96
Range % 98-100
1:8 Average % 90
Range % 85-94
Recovery :
The recovery of human CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 94 90-100
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
20 0.361 0.369 0.365
10 0.518 0.501 0.510
5 0.759 0.774 0.767
2 1.232 1.200 1.216
1 1.780 1.695 1.738
0.5 2.194 2.213 2.204
0 2.647 2.682 2.665

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