Description
CSB-E04647h, Human Leptin receptor,LR/Ob-R ELISA KIT, 96T,5×96T,10×96T
| Product Name: |
Human Leptin receptor,LR/Ob-R ELISA KIT
|
| Product Type : |
ELISA Kit |
| Code : |
CSB-E04647h |
| Size : |
96T,5×96T,10×96T |
| Uniprot NO. : |
P48357 |
| Abbreviation : |
LEPR |
| Alias : |
CD295, OBR, OB receptor|OTTHUMP00000010694 |
| Species : |
Human |
| Sample Types : |
serum, plasma, tissue homogenates |
| Detect Range : |
3.12 pg/ml-200 pg/ml |
| Sensitivity : |
0.78 pg/ml |
| Assay Time : |
1-5h |
| Sample Volume : |
50-100ul |
| Detection Wavelengt : |
450 nm |
| Availability : |
3-5 working days |
| Research Area : |
Metabolism |
| Protocol : |
Instructions
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| HGNC : |
6554 |
| RGD : |
3001 |
| MGI : |
104993 |
| BIOWWW : |
LEPR |
| Precision : |
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
|
|
|
|
|
|
|
| Linearity : |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LR/Ob-R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
90 |
|
| Range % |
86-93 |
|
| 1:400 |
Average % |
100 |
|
| Range % |
97-103 |
|
| 1:800 |
Average % |
94 |
|
| Range % |
89-97 |
|
| 1:1600 |
Average % |
98 |
|
| Range % |
94-101 |
|
|
| Recovery : |
| The recovery of human LR/Ob-R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
91-97 |
|
| EDTA plasma (n=4) |
97 |
92-99 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Typical Data : |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 200 |
2.600 |
2.584 |
2.592 |
2.469 |
|
| 100 |
2.319 |
2.222 |
2.271 |
2.148 |
|
| 50 |
1.725 |
1.711 |
1.718 |
1.595 |
|
| 25 |
1.099 |
1.068 |
1.084 |
0.961 |
|
| 12.5 |
0.628 |
0.644 |
0.636 |
0.513 |
|
| 6.25 |
0.382 |
0.393 |
0.388 |
0.265 |
|
| 3.12 |
0.243 |
0.257 |
0.250 |
0.127 |
|
| 0 |
0.122 |
0.124 |
0.123 |
|
|
|
|
| Reference : |
1. The Relation of Leptin and Soluble Leptin Receptor Levels with Metabolic and Clinical Parameters in Obese and Healthy Children,Catli G et al,Peptides,2014 Apr 2. pii: S0196-9781(14)00100-4.
|
Reviews
There are no reviews yet.