L1 medium is recommended for the culture of bioluminescent algae. L1 is an updated formulation of Guillard’s f/2 medium. The BMC cultures all bioluminescent algae on L1 medium.
Empco L1 Medium Technical Specifications:
The L1-Si (L1 minus Silica) enriched seawater medium is an updated formulation based on the f/2 medium (Guillard and Ryther 1962). L1-Si contains additional trace metals compared to f/2, and omits silica to reduce precipitation. It is a general purpose liquid marine medium for growth of coastal algae. Empco’s L1 medium is prepared at 2X nutrient concentration of the original formulation, and should be used undiluted for growth of Pyrocystis species. This medium may be used to replenish nutrients in actively growing cultures of bioluminescent algae. To use, combine 1 part algal culture with 5 parts growth medium, or as desired. Detailed instructions are included with purchase.